Description
This assay employs the competitive inhibition enzyme immunoassay technique. A monoclonal antibody specific to Inhibin A (INHA) has been pre-coated onto a microplate. A competitive inhibition reaction is launched between biotin labeled Inhibin A (INHA) and unlabeled Inhibin A (INHA) (Standards or samples) with the pre-coated antibody specific to Inhibin A (INHA). After incubation the unbound conjugate is washed off. Next, avidin conjugated to Horseradish Peroxidase (HRP) is added to each microplate well and incubated. The amount of bound HRP conjugate is reverse proportional to the concentration of Inhibin A (INHA) in the sample. After addition of the substrate solution, the intensity of color developed is reverse proportional to the concentration of Inhibin A (INHA) in the sample